Journal: PLOS One
Article Title: Ozone ultrafine bubble water sterilizes Porphyromonas gingivalis and neutralizes its virulence factors
doi: 10.1371/journal.pone.0334259
Figure Lengend Snippet: P. gingivalis LPS was added to distilled water (DW), air ultrafine bubble water (AUFBW), or 3.03 ppm ozone ultrafine bubble water (OUFBW). (A) HEK-Blue human Toll-like receptor 2 (hTLR2), (B) HEK-Blue hTLR4, and (C) HEK-Blue null2 cells were stimulated with the LPS. Pam3CSK4 (Pam), a TLR2 agonist, was used as the control. Secreted alkaline phosphatase (SEAP) levels were quantified using spectrophotometry at 620 nm. The data represent means ± SD of sextuplicate experiments and were evaluated by one-way analysis of variance with Tukey’s multiple comparisons test. †, significant difference compared to DW group at P < 0.05. *, significant difference between the indicated groups at P < 0.05.
Article Snippet: HEK-Blue cells expressing human TLR2 (HEK-hTLR2), HEK-hTLR4 and HEK-null2 cells (InvivoGen) were cultured at 37 °C in 5% CO 2 .
Techniques: Control, Spectrophotometry